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Sarstedt transwell polyester membrane inserts
Transwell Polyester Membrane Inserts, supplied by Sarstedt, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/transwell+polyester+membrane+inserts/inserts+transwell/pm41366543-198-8-33
Average 86 stars, based on 1 article reviews
transwell polyester membrane inserts - by Bioz Stars, 2026-10
86/100 stars

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Related Articles

Membrane:

Article Title: IPEC-J2 as a cellular model for studying intestinal mucus.
Article Snippet: The IPEC-J2, passages 3 to 6, were grown in 75 cm2 flasks 363 containing Dulbecco’s Modified Eagle Medium/Ham’s F-12 (DMEM/F12, Gibco, reference 364 31331028) supplemented with 10% porcine serum (PS) containing 1% penicillin-365 streptomycin (Gibco, reference 15140122) as previously described14. .. At 80% confluence, 366 cells were seeded on transwell polyester membrane inserts (0.4 μm pore size, 1.1 cm2 surface 367 area), placed in 12-well plates at a density of 1x105 cells per cm2 (Sarstedt, reference 368 2582608), and in DMEM/F12 medium supplemented with either 5% PS, 1% penicillin-369 streptomycin, 1% Insulin Transferrin Selenium (ITG, Gibco, reference 41400045), and 10 370 μg/mL Epithelial Growth Factor (EGF, Merck, reference SRP3027-500UG); or 10% PS and 371 1% penicillin-streptomycin. .. For these two conditions, 5 or 10% PS, the cells were culture 372 AR TIC LE IN PR ES S without (5PS and 10PS) or with orbital agitation (5PSAg and 10PSAg) at 200 rpm 373 (Microplate Mixer, N2400-8040, StarLab, Hamburg, Germany).

Article Title: IPEC-J2 as a cellular model for studying intestinal mucus
Article Snippet: The IPEC-J2, passages 3 to 6, were grown in 75 cm 2 flasks containing Dulbecco’s Modified Eagle Medium/Ham’s F-12 (DMEM/F12, Gibco, reference 31331028) supplemented with 10% porcine serum (PS) containing 1% penicillin-streptomycin (Gibco, reference 15140122) as previously described . .. At 80% confluence, cells were seeded on transwell polyester membrane inserts (0.4 μm pore size, 1.1 cm 2 surface area), placed in 12-well plates at a density of 1 × 10 5 cells per cm 2 (Sarstedt, reference 2582608), and in DMEM/F12 medium supplemented with either 5% PS, 1% penicillin-streptomycin, 1% Insulin Transferrin Selenium (ITG, Gibco, reference 41400045), and 10 μg/mL Epithelial Growth Factor (EGF, Merck, reference SRP3027-500UG); or 10% PS and 1% penicillin-streptomycin. .. For these two conditions, 5 or 10% PS, the cells were culture without (5PS and 10PS) or with orbital agitation (5PSAg and 10PSAg) at 200 rpm (Microplate Mixer, N2400-8040, StarLab, Hamburg, Germany).

Pore Size:

Article Title: IPEC-J2 as a cellular model for studying intestinal mucus.
Article Snippet: The IPEC-J2, passages 3 to 6, were grown in 75 cm2 flasks 363 containing Dulbecco’s Modified Eagle Medium/Ham’s F-12 (DMEM/F12, Gibco, reference 364 31331028) supplemented with 10% porcine serum (PS) containing 1% penicillin-365 streptomycin (Gibco, reference 15140122) as previously described14. .. At 80% confluence, 366 cells were seeded on transwell polyester membrane inserts (0.4 μm pore size, 1.1 cm2 surface 367 area), placed in 12-well plates at a density of 1x105 cells per cm2 (Sarstedt, reference 368 2582608), and in DMEM/F12 medium supplemented with either 5% PS, 1% penicillin-369 streptomycin, 1% Insulin Transferrin Selenium (ITG, Gibco, reference 41400045), and 10 370 μg/mL Epithelial Growth Factor (EGF, Merck, reference SRP3027-500UG); or 10% PS and 371 1% penicillin-streptomycin. .. For these two conditions, 5 or 10% PS, the cells were culture 372 AR TIC LE IN PR ES S without (5PS and 10PS) or with orbital agitation (5PSAg and 10PSAg) at 200 rpm 373 (Microplate Mixer, N2400-8040, StarLab, Hamburg, Germany).

Article Title: IPEC-J2 as a cellular model for studying intestinal mucus
Article Snippet: The IPEC-J2, passages 3 to 6, were grown in 75 cm 2 flasks containing Dulbecco’s Modified Eagle Medium/Ham’s F-12 (DMEM/F12, Gibco, reference 31331028) supplemented with 10% porcine serum (PS) containing 1% penicillin-streptomycin (Gibco, reference 15140122) as previously described . .. At 80% confluence, cells were seeded on transwell polyester membrane inserts (0.4 μm pore size, 1.1 cm 2 surface area), placed in 12-well plates at a density of 1 × 10 5 cells per cm 2 (Sarstedt, reference 2582608), and in DMEM/F12 medium supplemented with either 5% PS, 1% penicillin-streptomycin, 1% Insulin Transferrin Selenium (ITG, Gibco, reference 41400045), and 10 μg/mL Epithelial Growth Factor (EGF, Merck, reference SRP3027-500UG); or 10% PS and 1% penicillin-streptomycin. .. For these two conditions, 5 or 10% PS, the cells were culture without (5PS and 10PS) or with orbital agitation (5PSAg and 10PSAg) at 200 rpm (Microplate Mixer, N2400-8040, StarLab, Hamburg, Germany).



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